Configurable 3D‐Printed Microstructured Stamp as a User‐Friendly Tool for Versatile Patterning of Low‐Viscosity Bioinks

Abstract Microcontact printing is a widely used technique for controlling the spatial resolution of cells, particularly in cell patterning. While it is known for its high‐resolution capabilities, its accessibility and versatility are limited due to its reliance on cleanroom facilities and the requirement for surface modifications. Additionally, this technique is mainly suitable for 2D protein‐based patterning and faces challenges in adapting to hydrogel‐based biofabrication. Hydrogel‐based systems are commonly used to mimic the native extracellular matrix of tissues. Fibrin, a highly biocompatible hydrogel that supports endothelial cell activity, is difficult to pattern due to its weak mechanical stability and low viscosity, making it unsuitable for traditional bioprinting methods. In this study, a configurable 3D‐printed microstructured stamp is proposed, enabling easy‐to‐use cell patterning with a modular stamping setup. Unlike conventional microcontact printing methods associated with photolithography and stamping, the approach enables rapid and customizable fabrication of micropillar‐based stamps that effectively trap and transfer low‐viscosity bioinks. Multiple bioinks can also be patterned using different types of microstructured stamps. The microstructured stamp is expected to become a robust and easy‐to‐use platform for controlling the spatial resolution of bioink for in vitro models for biomedical applications.