Polydopamine-engineered FRET microspheres for robust dual-readout multiplexed nucleic acid analysis
We present a polydopamine-engineered FRET-encoded microsphere platform that ensures stable spectral encoding, efficient decoding, improved dispersion, and reproducible high-density DNA immobilization (∼0.1 nmol mg<sup>-1</sup>), enabling robust and sensitive multiplexed nucleic acid analysis with low background signals.
Authors
- Xinrui He (ORCID: https://orcid.org/0000-0002-9139-2008)
- Liwen Tang
- Yuanqing Zhu (ORCID: https://orcid.org/0000-0002-9481-4165)
- Dongzhi Yang (ORCID: https://orcid.org/0000-0001-6796-1613)
- Chao Guo (ORCID: https://orcid.org/0000-0002-7929-822X)
- Jing Chen (ORCID: https://orcid.org/0009-0008-9227-0396)
- Jie Song (ORCID: https://orcid.org/0000-0003-1635-9598)
- Yu Guo (ORCID: https://orcid.org/0000-0001-9397-1858)
- Yi Chen (ORCID: https://orcid.org/0000-0002-6310-9762)
Institutions
- Xuzhou Medical College (CN)
- Chinese Academy of Sciences (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc03811a
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Xuzhou Medical University
- Major Basic Research Project of the Natural Science Foundation of the Jiangsu Higher Education Institutions