FluMag-SELEX derived specific individual aptamers can fluorescently label the ageing immanent protein-crosslinker glucosepane in diabetic mouse tissues
Nowadays, aptamers have transitioned into valuable antibody alternatives. We present the first anti-glucosepane aptamer targeting a key glycation product linked to aging and diabetes. Glu3, with high specificity and affinity, enabled the first-ever direct, fluorescence-based histological staining of glucosepane in murine tissue, distinguishing diabetic samples from wild-type samples without secondary reagents.
Authors
- Karmveer Singh (ORCID: https://orcid.org/0000-0002-1237-7417)
- Frank Rosenau (ORCID: https://orcid.org/0000-0002-9297-6419)
- Mika Lindén (ORCID: https://orcid.org/0000-0003-1736-0638)
- Karin Scharffetter‐Kochanek (ORCID: https://orcid.org/0000-0002-9655-685X)
- Ann‐Kathrin Kissmann (ORCID: https://orcid.org/0000-0002-7164-0166)
- Bastian Draphoen (ORCID: https://orcid.org/0009-0004-6007-1473)
- Runliu Li
- Andreas Stürmer
Institutions
- Danube Private University (AT)
- Universität Ulm (DE)
- GP Forschungsgruppe (DE)
Publication Details
- Journal
- Nanoscale Horizons
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5nh00263j
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Deutsche Forschungsgemeinschaft
- China Scholarship Council
- Österreichische Forschungsförderungsgesellschaft