Investigation of antibiofilm activity of bacteriophage ETEC-phage-TG and BC-VP against pathogenic Escherichia coli biofilms

Bacteriophages isolated from previous research have the potential to degrade biofilm. Bacteriophage ETEC-Phage-TG have titer 4.50 × 10⁵ PFU/mL towards EHEC and a Minimum Inhibitory Multiplicity of Infection of 0.01 (10^{- 2}). Bacteriophage BC-VP had titer 8.0877 × 10⁴ PFU/mL towards EPEC and a Minimum Inhibitory Multiplicity of Infection of 0.001 (10^{- 3}). The bacteriophage ETEC-phage-TG was able to inhibit and destruct biofilm formation on polystyrene surface at 25.43% and 11.41% respectively. Bacteriophage ETEC-phage-TG was able to destruct biofilm formation (24 h and 48 h) on stainless steel surface at 37.87% and 39.21% respectively. Bacteriophage BC-VP was able to inhibit and destruct biofilm on polystyrene surface at 54.57% and 26.36% respectively, it was also able to destruct biofilm formation (24 h and 48 h) on stainless steel surface 33.21% and 43.94% respectively.