Investigation of an Injectable Concentrated Growth Factor Gel for Pulp Tissue Regeneration

Cell homing and stem cell transplantation offers promising approaches for pulp regeneration. This study investigated the efficacy of an injectable concentrated growth factor gel for dental pulp regeneration in beagles. Six groups of beagle teeth were studied: Blank control group, CGF group, Collagen sponge group, CGF + Collagen sponge group, Dental pulp stem cells group, CGF + Dental pulp stem cells group. Specimens were examined by CBCT and histological analysis. No obvious change was identified in the periapical condition. Fewest inflammatory cells were found in the CGF + CS group. Odontoblast-like cells and dentin-like tissue were identified in the root canals of experimental groups, and the thickness of dentin-like tissue was positively correlated with the number of odontoblast-like cells. CD31-positive cells appeared in root canals containing pulp-like tissue. Injectable concentrated growth factor (CGF) can induce in situ pulp-dentin complex regeneration either on its own or in combination with collagen sponge and dental pulp stem cells.