Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to detect newly synthesized proteins in cells and their secretome
Our work demonstrates the power of a modified BONCAT protocol to study newly produced proteins in growing cells and their secretome. This method will be useful to examine the proteome and secretome changes that are linked to the altered performance of cells, tissues, and organs during aging, disease, or other challenging conditions.
Authors
- Ursula Stochaj (ORCID: https://orcid.org/0000-0003-4040-8695)
- Siwei Chu (ORCID: https://orcid.org/0000-0002-2957-9285)
- Elizabeth P. Anim
- Justin Mezzanotte
Institutions
- McGill University (CA)
Publication Details
- Journal
- PLoS ONE
- Published
- 2025-08-14
- DOI
- https://doi.org/10.1371/journal.pone.0329857
- Citations
- 1
- Primary Topic
- Cancer Research and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 1.23