Real-time monitoring of secretory protein traffic for investigating trafficking regulators of ACE2 in living cells
The cellular secretory pathway is a fundamental physiological process; yet a precise, efficient, and quantitative method for monitoring this process remains seldom explored. In this study, we achieved real-time monitoring of the secretion of angiotensin-converting enzyme 2 and programmed death-ligand 1 by integrating the HiBiT tag with the classic Retention Using Selective Hooks system. Through this strategy, we identified and validated Niemann-Pick complex 1 as a lysosomal adaptor involved in the angiotensin-converting enzyme 2 trafficking process. We propose that the strategy offers a valuable tool for the precise and rapid identification of diverse trafficking regulators and therapeutic compounds targeting various receptors or cytokines, thereby advancing the field of cellular transport research.
Authors
- Linfei Huang
- Xiaopan Yang (ORCID: https://orcid.org/0000-0003-1442-6179)
- Ruzhou Zhao
- Xinlong Yan (ORCID: https://orcid.org/0000-0001-9967-5654)
- Muyi Liu (ORCID: https://orcid.org/0000-0003-2623-9311)
- Congwen Wei (ORCID: https://orcid.org/0000-0002-3966-5507)
- Luming Wan (ORCID: https://orcid.org/0000-0002-0698-4077)
- Chang Zhang (ORCID: https://orcid.org/0000-0001-5668-4649)
- Yanhong Zhang (ORCID: https://orcid.org/0000-0002-8421-9004)
- Renjun Peng
- Yu An (ORCID: https://orcid.org/0000-0002-0373-3272)
- Hui Zhong (ORCID: https://orcid.org/0000-0001-6776-5532)
- Mengxin Yang (ORCID: https://orcid.org/0009-0002-4932-448X)
- Huilong Li (ORCID: https://orcid.org/0009-0007-0192-0692)
Institutions
- Beijing Normal University (CN)
- Beijing University of Technology (CN)
- Beijing VDJBio (China) (CN)
- Beijing Biocytogen (China) (CN)
- Czech Academy of Sciences, Institute of Biotechnology (CZ)
- BeiGene (China) (CN)
- Beijing Municipal Ecological and Environmental Monitoring Center (CN)
- University of Science and Technology Beijing (CN)
- Capital Normal University (CN)
Publication Details
- Journal
- Journal of Biological Chemistry
- Published
- 2025-08-12
- DOI
- https://doi.org/10.1016/j.jbc.2025.110593
- Primary Topic
- Cellular transport and secretion
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China