Analytical comparison of 11 CE-IVD marked real-time PCR assays for Borrelia burgdorferi sensu lato detection
This study shows that the analytical performance and practicability of the kits evaluated are broadly in line, but that advanced molecular biology skills are required to interpret the results. Simultaneous detection of Lyme Borreliosis and RF agents without precise distinction may result in a risk of confusion in the choice of biological matrices and in the wording of the result returned to the clinician. Diagnostic laboratories must pay particular attention to the amplification confirmation strategy.
Authors
- Émilie Talagrand-Reboul (ORCID: https://orcid.org/0000-0001-9767-0753)
- Maximilian Faller
- Laurence Zilliox
- Gabriele Margos (ORCID: https://orcid.org/0000-0001-5304-9615)
- Volker Fingerle (ORCID: https://orcid.org/0000-0002-3835-5646)
- Lisa Baldinger (ORCID: https://orcid.org/0000-0003-1053-6311)
- Barbara Streibl (ORCID: https://orcid.org/0000-0001-9644-1609)
- B. Jaulhac
- Marguerite Engel
Institutions
- Institut National des Sciences Appliquées de Strasbourg (FR)
- Institut de Virologie (FR)
- Hôpitaux Universitaires de Strasbourg (FR)
- Bayerisches Landesamt für Gesundheit und Lebensmittelsicherheit (DE)
- Université de Strasbourg (FR)
Publication Details
- Journal
- European Journal of Clinical Microbiology & Infectious Diseases
- Published
- 2025-08-14
- DOI
- https://doi.org/10.1007/s10096-025-05214-5
- Citations
- 2
- Primary Topic
- Vector-borne infectious diseases
- Type
- article
- Field-Weighted Citation Impact
- 4.10
Funders
- Université de Strasbourg
- Agence Nationale de Sécurité du Médicament et des Produits de Santé