The Effect of Dimethyl Fumarate on Tissues in Methanol Poisoning

Objective: This study aimed to investigate the effects of an antioxidant, dimethyl fumarate (DMF), on methanol (MeOH)-induced liver, kidney, testicular, and ocular toxicity in Wistar rats.Methods: Six experimental groups were established: a control group; a group receiving (DMF; 100 mg/kg); a group receiving methotrexate [(MTX); 0.3 mg/kg/day]; and three groups receiving MTX followed by MeOH (3 g/kg, oral, 20% solution) with either 30 mg/kg or 100 mg/kg DMF, or no DMF.MTX was administered for seven days prior to MeOH, and DMF was administered two hours before MeOH, on the eighth day.Animals were sacrificed eight hours after MeOH administration.Results: Minimal changes were seen in the urine test, such as a high protein score and increased pH value in the MTX + MeOH group, and a significant level of bilirubin excretion in the MTX + MeOH + DMF100 group.Only the liver weight/body weight X100 index was lower in the DMF group.There were no histopathological changes in liver, kidney, testicular, and eye tissues.Conclusion: DMF was found to be safe for the liver, kidney, testicular, and ocular tissues.However, it was not possible to achieve the MeOH toxicity model in the specified doses and routes in the rats, contrary to the related literature.Future studies are needed to develop a reliable model for MeOH toxicity in rats.