Different Methods of Olive Micropropagation

Micropropagation is an efficient method for rapid asexual propagation, production of disease-free plants, and access to propagating materials throughout the year for olive plants. In this study, with the aim of investigation different methods of olive micropropagation, explants of Kroneiki cultivar from olive trees were cultured on different basal culture media, MS (Murashige and Skoog), OM (Olive Medium), DKW (Driver and Kuniyki), and WPM (Woody Plant Medium) without plant growth regulators. Then, the explants were cultured on basal culture media enriched with auxins NAA (Naphthalene acetic acid, IAA (Indole acetic acid) and IBA (Indole butyric acid)) and cytokinins BA (Benzyl adenine), KIN (Kinetin), 2ip (Isopentenyl adenine), TDZ (Thidiazuron), and ZEA (Zeatin) at concentrations of 0.05 to 7.38 μM/L. The superiority of DKW basal culture medium and ZEA growth regulator for shoot induction and longitudinal growth of induced shoot was proven. With increasing concentration of growth regulators, especially BA, the callus reaction was dominant over other reactions. Different concentrations of growth regulators NAA, IAA and IBA (0.05 to 8 μM/L) were used for rooting which was often unsuccessful. Finally, the rooting stage of the regenerated shoots was achieved by soaking the shoots (the basal part of the shoots) in NAA solution (37.5 μM/L for 5 minutes) and transferring them directly to the soil.