Evaluation of diagnostic accuracy of Allergy Explorer 2 method in children with bronchial asthma and allergic rhinitis in the Kyrgyz Republic

Objective: The evaluate efficacy and the diagnostic accuracy of Allergy Explorer 2 (ALEX-2) test system in pediatric patients with bronchial asthma (BA) and allergic rhinitis (AR) in conditions of limited access to skin tests in Kyrgyz Republic. Methods: Overall, 60 children with clinically confirmed BA and/or AR were included in the study. Molecular allergy diagnostics using the ALEX-2 test system was conducted for patients in the study cohort. The methodology included serum analysis to evaluate total IgE levels and detect specific to IgE 295 allergens. Results: The mean total serum IgE level was 1255.37 (150.43) kUA/L. Boys exhibited significantly higher IgE concentrations compared to girls (p<0.01). A high degree of sensitization was identified to pollen allergens, epidermal allergens and domestic common allergens. ALEX-2 test allowed individualizing allergy diagnostics and facilitating precise indications for allergen-specific immunotherapy (ASIT). Conclusions:. ALEX-2 testing showed high clinical significance in the absence of standard skin tests. Further studies with inclusion of comparative methods of diagnostics are required.