Confocal versus interference microscopy: 3D resolution analysis

Confocal and interference microscopy are the two most widely used optical techniques for surface topography measurement. Nonetheless, due to diffraction effects, which are inevitable in optical far-field measurement methods, both techniques are limited in their lateral resolution. In contrast to conventional microscopes, where the lateral resolution is usually defined by a single number such as the Abbe limit, a more comprehensive (3D) characterization of resolution is required for surface topography measurement. The instrument transfer function (ITF) provides the height response of a profiler as a function of the surface’s lateral spatial frequency content assuming linear systems theory. Comparing numerically calculated ITFs, we outline that interference microscopy can outperform confocal microscopy in terms of both, lateral and axial resolution, provided that appropriate signal processing is being used.

Authors

Institutions

Publication Details

Published
2025-08-08
DOI
https://doi.org/10.1117/12.3060501
Primary Topic
Advanced Fluorescence Microscopy Techniques
Type
article
Field-Weighted Citation Impact
0.00
Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Confocal versus interference microscopy: 3D resolution analysis

Peter Lehmann, Tobias Pahl, Yijian Zou, F. Rosenthal
Advanced Fluorescence Microscopy Techniques
article

Confocal versus interference microscopy: 3D resolution analysis

Peter Lehmann, Tobias Pahl, Yijian Zou, F. Rosenthal
article en

Abstract

Confocal and interference microscopy are the two most widely used optical techniques for surface topography measurement. Nonetheless, due to diffraction effects, which are inevitable in optical far-field measurement methods, both techniques are limited in their lateral resolution. In contrast to conventional microscopes, where the lateral resolution is usually defined by a single number such as the Abbe limit, a more comprehensive (3D) characterization of resolution is required for surface topography measurement. The instrument transfer function (ITF) provides the height response of a profiler as a function of the surface’s lateral spatial frequency content assuming linear systems theory. Comparing numerically calculated ITFs, we outline that interference microscopy can outperform confocal microscopy in terms of both, lateral and axial resolution, provided that appropriate signal processing is being used.

University of Kassel (DE)
Openalex Percentile: Top 85%
Advanced Fluorescence Microscopy Techniques
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.