DETERMINATION OF 3,4 DIHYDROXY BENZOIC ACID FROM “BAWANG HUTAN” Scorodocarpus borneensis Becc. AS AN ANTIOXIDANT

Forest onion (Bawang hutan, local name), Scorodocarpus borneensis Becc is one of the medicinal ingredients considered to have properties that are useful as antioxidants, anticancer, antibacterial, antifungal, etc. This plant is widely found in the forest areas of Kalimantan, Indonesia. While its cultivation is still very lacking. In this research, the chopped bark of bawang hutan was refluxed with methanol solvent, then the methanol extract was partitioned to ethyl-acetate: water = 1:1. The ethyl acetate phase obtained was fractionated by column chromatography (SiO2; i). n-hexan : ethyl acetate = 20:1~2:1; ii). n-hexane-ethylacetate = 5: 1; and preparative thin layer chromatography (n-hexaneethylacetate = 2:1). Each fraction was tested for antioxidant activity by the free radical scavenging method using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and obtained pure isolates that have antioxidant activity in fraction SbEA 8-5. Based on the interpretation of Ultra-violet-visible (UV-Vis), Fourier Transform Infra Red (FT-IR) spectra, Nuclear Magnetic Resonance (NMR) 1D 1H, 13C-NMR, DEPT, and NMR 2D (HMQC, COSY, HMBC), the chemical compound is 3,5-dihydroxybenzoic acid (protocatechuic acid) which has an IC50 of 12.05 ppm.