Resolution test of STED microscopy using nanodiamonds with embedded nitrogen-vacancy centers
We investigated the suitability of nanodiamonds (NDs) with nitrogen-vacancy (NV) centres as samples for resolution tests in stimulated emission depletion (STED) microscopy. The nanodiamonds were prepared using three different methods including additional proton irradiation to enhance NV centre density. This high-density sample exhibited stronger photoluminescence intensity, greater fluorescence stability, and higher image contrast under STED conditions. All samples showed strong resistance to damage from high STED laser power. Our results highlight the robustness and effectiveness of NDs with NV centres as reliable standards for evaluating STED microscope resolution.
Authors
- Stefanie Kroker (ORCID: https://orcid.org/0000-0002-7584-7359)
- Bernd Bodermann (ORCID: https://orcid.org/0000-0001-8297-4068)
- P. Olivero (ORCID: https://orcid.org/0000-0002-7512-6295)
- Pietro Aprà (ORCID: https://orcid.org/0000-0002-0973-3775)
- Mohammad Nouri (ORCID: https://orcid.org/0000-0002-4782-9305)
Institutions
- Physikalisch-Technische Bundesanstalt (DE)
- Istituto Nazionale di Fisica Nucleare (IT)
- University of Turin (IT)
- Technische Universität Braunschweig (DE)
Publication Details
- Published
- 2025-08-08
- DOI
- https://doi.org/10.1117/12.3062227
- Primary Topic
- Diamond and Carbon-based Materials Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00