Electrochemical Analysis Reveals the Chemiluminescence Processes of Coelenterazine to Deprotonated Coelenteramide

Coelenterazine is the most common imidazopyrazinone‐based luciferin in marine bioluminescence organisms. The electrochemical main peaks of coelenterazine are observed at approximately around 0.12 and 0.62 Vs in the cyclic voltammetry curve. Theoretical analysis suggests that coelenterazine is converted to the coelenteramide around 0.12 V, and the coelenteramide is deprotonated at around 0.62 V. These approaches can reveal the possible excited states of coelenteramide but do not consistently enable the enzymatic bioluminescence reaction to be traced.