Protocol to generate genetically engineered organoid-initiated mouse models of esophageal cancer
Here, we present a protocol for generating genetically engineered organoid-initiated mouse models of esophageal cancer. We describe steps for isolating mouse esophageal primary cells, organoids culture, construct and virus preparation, and genome editing. We then detail procedures for transplanting engineered organoids into the esophagus, forming squamous cell carcinoma, and tumorigenesis monitoring. The protocol is faster than genetically engineered mouse models. For complete details on the use and execution of this protocol, please refer to Wang et al.<sup>1</sup>.
Authors
- Yong Yuan (ORCID: https://orcid.org/0000-0001-9720-1965)
- Yu‐Shang Yang (ORCID: https://orcid.org/0000-0001-5100-2266)
- Xudong Wan
- Bing Hu (ORCID: https://orcid.org/0000-0002-9898-8656)
- Zhenghao Lu
- Feifei Na
- Longqi Chen (ORCID: https://orcid.org/0000-0002-5036-5345)
- Yanhong Ji (ORCID: https://orcid.org/0000-0003-2782-6825)
- Ping Tan (ORCID: https://orcid.org/0000-0001-5916-5588)
- Yu Liu (ORCID: https://orcid.org/0009-0007-1970-8614)
- He Li (ORCID: https://orcid.org/0000-0001-9139-0873)
- Chong Chen (ORCID: https://orcid.org/0000-0002-6787-0495)
- Lu Wang (ORCID: https://orcid.org/0000-0003-2617-655X)
- Jian Wang (ORCID: https://orcid.org/0000-0002-9482-5227)
- Yaxin Li (ORCID: https://orcid.org/0000-0003-3205-3740)
- Yuan Li (ORCID: https://orcid.org/0000-0002-5969-708X)
- Xiaoling Luo (ORCID: https://orcid.org/0009-0004-9640-7899)
- Xiang Li (ORCID: https://orcid.org/0000-0003-4008-2541)
- Zhongxun Dong (ORCID: https://orcid.org/0000-0002-0061-2846)
- Jiajia Du (ORCID: https://orcid.org/0009-0002-5235-7629)
- Linjie Guo
- Hongyu Liu (ORCID: https://orcid.org/0000-0003-0383-8725)
Institutions
- Sichuan University (CN)
- West China Medical Center of Sichuan University (CN)
- West China Hospital of Sichuan University (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-08-12
- DOI
- https://doi.org/10.1016/j.xpro.2025.104028
- Primary Topic
- Neonatal Respiratory Health Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00