An adjuvant database for preclinical evaluation of vaccines and immunotherapeutics
Adjuvants are immunostimulators used to enhance vaccine efficacy against infectious diseases. However, current methods for evaluating their efficacy and safety are limited, hindering large-scale screening. To address this, we developed a prototype Adjuvant Database (ADB) containing transcriptome data, generated using the same protocols as the widely used Open TG-GATEs (OTG) toxicogenomics database, covering 25 adjuvants across multiple species, organs, time points, and doses. This enabled cross-database integration of ADB and OTG. Transcriptomic patterns successfully distinguished each adjuvant regardless of organs or species. Using both databases, we built machine learning models to predict adjuvanticity and hepatotoxicity. Notably, we identified colchicine's adjuvant activity and FK565's liver toxicity through data-driven analysis. Overall, ADB combined with OTG offers a framework for transcriptomics-based, data-driven screening of adjuvant candidates.
Authors
- Daron M. Standley (ORCID: https://orcid.org/0000-0003-4078-0817)
- Yuji Kosugi
- Jun‐ichi Ito (ORCID: https://orcid.org/0000-0003-0673-9474)
- Johan Nyström-Persson (ORCID: https://orcid.org/0000-0002-7951-4762)
- Burcu Temizoz (ORCID: https://orcid.org/0000-0002-5189-3529)
- Cevayir Coban (ORCID: https://orcid.org/0000-0002-4467-7799)
- Hiroshi Yamada (ORCID: https://orcid.org/0000-0001-6678-2701)
- Taiki Aoshi (ORCID: https://orcid.org/0000-0003-1607-0639)
- Rodolfo S. Allendes Osorio (ORCID: https://orcid.org/0000-0002-9627-9338)
- Chioko Nagao (ORCID: https://orcid.org/0000-0002-7721-0642)
- Kenji Nakanishi (ORCID: https://orcid.org/0000-0002-0223-9088)
- Satoshi Uematsu (ORCID: https://orcid.org/0000-0002-5347-6737)
- Yoshinobu Igarashi (ORCID: https://orcid.org/0000-0001-8636-796X)
- Etsushi Kuroda (ORCID: https://orcid.org/0000-0002-7198-2190)
- Kenji Mizuguchi (ORCID: https://orcid.org/0000-0003-3021-7078)
- Lokesh P. Tripathi (ORCID: https://orcid.org/0000-0001-5812-2252)
- Ken J. Ishii (ORCID: https://orcid.org/0000-0002-6728-3872)
- Yayoi Natsume‐Kitatani (ORCID: https://orcid.org/0000-0003-1749-3318)
- Yasuhiro Yasutomi (ORCID: https://orcid.org/0000-0001-8252-1448)
- Noriyuki Nakatsu
- Kouji Kobiyama (ORCID: https://orcid.org/0000-0002-5846-4807)
- Jun Kunisawa (ORCID: https://orcid.org/0000-0002-6237-3289)
- Hiroshi Kiyono (ORCID: https://orcid.org/0000-0002-5150-1011)
- Isao Hamaguchi
- Sho Yamasaki
Institutions
- Hyogo Medical University (JP)
- National Institute of Infectious Diseases (JP)
- National Institute of Biomedical Innovation, Health and Nutrition (JP)
- Chiba University Hospital (JP)
- Protein Research Foundation (JP)
- Medidata (United States) (US)
- Nagasaki University (JP)
- Eisai (Japan) (JP)
- Tokyo Metropolitan University (JP)
- The University of Tokyo (JP)
- Hyogo University (JP)
- Tokushima University (JP)
- Chuo University (JP)
- The University of Osaka (JP)
Publication Details
- Journal
- Cell chemical biology
- Published
- 2025-08-01
- DOI
- https://doi.org/10.1016/j.chembiol.2025.07.005
- Citations
- 1
- Primary Topic
- interferon and immune responses
- Type
- article
- Field-Weighted Citation Impact
- 0.80