Novel Self-Powered Biosensor Based on Highly Efficient Cosignal Accelerator and Multiple Signal Amplification Strategies toward Ultrasensitive miRNA-141 Assay
A highly sensitive self-powered biosensor is designed based on gold-platinum nanorods (AuPt NRs) and the cascade reaction of catalytic hairpin assembly (CHA) and hybrid chain reaction (HCR) toward the miRNA-141 assay. As a cosignal accelerator, AuPt NRs enhance electrical conductivity between glucose oxidase (GOD) and a carbon paper (CP) electrode, thereby assisting in output signal enhancement. The cascade reaction of CHA-HCR is employed to efficiently amplify the detection signal and improve the sensitivity of the self-powered biosensor. Consequently, the self-powered biosensor provides highly sensitive detection of miRNA-141 with a wide linear response in the range of 0.1-10<sup>4</sup> fM and exhibits a low limit of detection (LOD) of 0.034 fM (<i>S/N</i> = 3). In addition, the self-powered biosensor exhibits high applicability in a real sample for the miRNA-141 assay.
Authors
- Yifan Lyu (ORCID: https://orcid.org/0000-0001-7314-7351)
- Weihong Tan (ORCID: https://orcid.org/0000-0002-8066-1524)
- Dan Yang (ORCID: https://orcid.org/0000-0001-6099-472X)
- Futing Wang (ORCID: https://orcid.org/0000-0001-7306-5495)
- Jingxian Li (ORCID: https://orcid.org/0000-0003-1087-5428)
- Yujin Li (ORCID: https://orcid.org/0000-0003-1103-3273)
- Hongfen Yang (ORCID: https://orcid.org/0000-0001-8367-4104)
- Ren Cai (ORCID: https://orcid.org/0000-0003-2583-7133)
- Yan Shen (ORCID: https://orcid.org/0000-0002-9744-3224)
Institutions
- Xinyang Normal University (CN)
- Hunan University (CN)
- Shanghai Jiao Tong University (CN)
- Chinese Academy of Sciences (CN)
- Renji Hospital (CN)
- MIT University (MK)
- Zhejiang Cancer Hospital (CN)
- Cancer Hospital of Chinese Academy of Medical Sciences (CN)
- Institute of Molecular Medicine (IN)
- Hengyang Academy of Agricultural Sciences (CN)
- University of Chinese Academy of Sciences (CN)
- RMIT University (AU)
- University of South China (CN)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2025-08-12
- DOI
- https://doi.org/10.1021/acs.analchem.5c03288
- Citations
- 5
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 4.03
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Hunan Province