Hepatic metabolism of 11-oxygenated androgens in humans: an integrated <i>in vivo</i> and <i>ex vivo</i> approach
Using in vivo and ex vivo approaches, we report the predominance of 11β-hydroxy metabolites, highlighting the pivotal role of hepatic 11β-hydroxysteroid dehydrogenase type 1 (HSD11B1) activity in 11-oxygenated androgen metabolism. We identify 11KAn as the only metabolite without overlap with glucocorticoid metabolism, underscoring its specificity and biomarker potential. NMLP represents a novel integrated model to study human hepatic steroid metabolism.
Authors
- Angus Hann (ORCID: https://orcid.org/0000-0003-4431-3642)
- Jingwen Mao (ORCID: https://orcid.org/0000-0001-7467-8461)
- Leanne Cussen (ORCID: https://orcid.org/0000-0002-9062-7696)
- Mark Sherlock (ORCID: https://orcid.org/0000-0003-4078-624X)
- Yiyu Fan
- Wiebke Arlt (ORCID: https://orcid.org/0000-0001-5106-9719)
- Marie McIlroy (ORCID: https://orcid.org/0000-0002-0827-8096)
- Lina Schiffer (ORCID: https://orcid.org/0000-0001-8540-4861)
- Angela E. Taylor (ORCID: https://orcid.org/0000-0002-5835-5643)
- Karl‐Heinz Storbeck (ORCID: https://orcid.org/0000-0003-1669-6383)
- Tara McDonnell (ORCID: https://orcid.org/0000-0002-6887-8333)
- Fozia Shaheen (ORCID: https://orcid.org/0000-0002-8123-4209)
- Clare Miller (ORCID: https://orcid.org/0000-0003-0193-8976)
- Simon C. Afford (ORCID: https://orcid.org/0000-0003-2858-3319)
- George R. G. Clarke (ORCID: https://orcid.org/0000-0002-9764-2987)
- Benson Jacob (ORCID: https://orcid.org/0000-0002-6663-2815)
- Michael O’Reilly (ORCID: https://orcid.org/0000-0002-9108-9105)
- Amarah Anthony
Institutions
- Royal College of Surgeons in Ireland (IE)
- Stellenbosch University (ZA)
- University of Medicine and Health Sciences (KN)
- General Medical Council (GB)
- Beaumont Hospital (IE)
- NIHR Birmingham Liver Biomedical Research Unit (GB)
- NIHR Imperial Biomedical Research Centre (GB)
- NIHR Birmingham Biomedical Research Centre (GB)
- Dublin City University (IE)
- University of Birmingham (GB)
- Medical Research Council (GB)
Publication Details
- Journal
- European Journal of Endocrinology
- Published
- 2025-08-08
- DOI
- https://doi.org/10.1093/ejendo/lvaf166
- Citations
- 2
- Primary Topic
- Hormonal and reproductive studies
- Type
- article
- Field-Weighted Citation Impact
- 2.59
Funders
- Wellcome Trust
- Health Research Board
- National Research Foundation
- Medical Research Council