Amorphous‐Crystalline Synergy in CoSe<sub>2</sub>/CoS<sub>2</sub> Heterostructures: High‐Performance SERS Substrates for Esophageal Tumor Cell Discrimination
Although surface-enhanced Raman scattering (SERS) spectroscopy is applied in biomedicine deeply, the design of new substrates for wider detection is still in demand. Crystalline-amorphous CoSe<sub>2</sub>/CoS<sub>2</sub> heterojunction is synthesized, with high SERS performance and stability, composed of orthorhombic (o-CoSe<sub>2</sub>) and amorphous CoS<sub>2</sub> (a-CoS<sub>2</sub>). By adjusting feed ratio, the proportion of a-CoS<sub>2</sub> to o-CoSe<sub>2</sub> is regulated, where CoSe<sub>2</sub>/CoS<sub>2</sub>-S50 with a 1:1 ratio demonstrates the best SERS performance due to the balance of two components. It is confirmed through experimental and simulation methods that o-CoSe<sub>2</sub> and a-CoS<sub>2</sub> have unique contribution, respectively: a-CoS<sub>2</sub> has rich vacancies and a higher density of active sites, while o-CoSe<sub>2</sub> further enriches vacancies, enhances electron delocalization and charge transfer (CT) capabilities, and reduces bandgap. Besides, CoSe<sub>2</sub>/CoS<sub>2</sub>-S50 achieves not only SERS detection of two common esophageal tumor cells (KYSE and TE) and healthy oral epithelial cells (het-1A), but also the discrimination with high sensitivity, specificity, and accuracy via machine learning (ML) analysis.
Authors
- Xiangyu Meng (ORCID: https://orcid.org/0000-0001-6740-5975)
- Aiguo Wu (ORCID: https://orcid.org/0000-0001-7200-8923)
- Jian Yu (ORCID: https://orcid.org/0000-0003-4561-552X)
- Ningli Chai (ORCID: https://orcid.org/0000-0002-6791-5817)
- Xiaoyu Song (ORCID: https://orcid.org/0000-0002-2986-8925)
- Mingjian Zhang (ORCID: https://orcid.org/0009-0006-6514-5317)
- Aochi Liu
- Yuening Wang (ORCID: https://orcid.org/0000-0003-4629-9872)
- Li Ning Sun (ORCID: https://orcid.org/0000-0001-8960-8773)
- Yu Sun (ORCID: https://orcid.org/0000-0001-8414-5159)
- Jie Lin (ORCID: https://orcid.org/0000-0003-4716-4689)
- Li Li (ORCID: https://orcid.org/0000-0003-4066-2298)
- Jiahao Zhang (ORCID: https://orcid.org/0000-0001-7083-3778)
- Xiaotian Wang (ORCID: https://orcid.org/0000-0002-1337-4875)
- Lei Xu (ORCID: https://orcid.org/0000-0003-4989-3623)
- Hao Liu (ORCID: https://orcid.org/0000-0001-5179-7337)
Institutions
- Hebei Medical University (CN)
- Chinese Academy of Sciences (CN)
- Chinese PLA General Hospital (CN)
- Ningbo Institute of Industrial Technology (CN)
- Hebei University (CN)
- Beihang University (CN)
- Nanchang Hangkong University (CN)
Publication Details
- Journal
- Small
- Published
- 2025-08-12
- DOI
- https://doi.org/10.1002/smll.202507530
- Citations
- 2
- Primary Topic
- Advanced Nanomaterials in Catalysis
- Type
- article
- Field-Weighted Citation Impact
- 1.04
Funders
- National Natural Science Foundation of China
- National Science and Technology Major Project
- Youth Innovation Promotion Association