Single cell labeling combined with electrophysiological identification

Gaining insight into the circuit architecture of neural tissue significantly aids in the investigation of nervous system mechanisms. Since neural networks are constituted by connections between axons and dendrites derived from neurons, morphological analysis of each neuron is useful to reconstruct circuit diagrams of neural circuits in detail. Here, I introduce a method where we can combine single unit recordings and visualization of neuronal morphology at a single neuron level. This technique reveals morphological characteristics of neurons which are electrophysiologically identified.