Retinal transduction profiling of diverse AAV serotypes via intravitreal injection

The retinal transduction efficiency and cellular tropisms of serial AAV serotypes, including AAV2, AAV2.7m8, AAV2.NN, AAV2.GL, AAV8, AAV11, and AAV.SPR, were simultaneously and unbiasedly quantified and compared following intravitreal injection. Transgene fluorescence was detectable in cells as early as 3 days post-injection in retinas intravitreally transduced with both ssAAV2.NN and scAAV2.NN vectors. The timeliness of the onset and level of transgene expression in retinas intravitreally transduced with ssAAV2.NN and scAAV2.NN vectors were characterized during the early phase post-injection. Differences in retinal transduction efficiency and cellular tropisms of scAAV2.NN vectors at varying doses via intravitreal injection are described.