Deletion of rgaS reduces virulence in a mouse model of Clostridioides difficile infection
Two-component system signalling is involved in several processes relevant to Clostridioides difficile virulence. We demonstrate that deletion of the orphan histidine kinase rgaS (R20291_0503), reduces detectable toxin in the supernatant of R20291 and impairs virulence in a mouse model of infection. These data suggest that RgaS regulates virulence capabilities in hypervirulent C. difficile.
Authors
- Terry W. Bilverstone (ORCID: https://orcid.org/0000-0001-7176-6917)
- Morgan Simpson (ORCID: https://orcid.org/0000-0003-3004-4517)
- Sarah A. Kuehne (ORCID: https://orcid.org/0000-0001-6790-8433)
- Nigel P. Minton (ORCID: https://orcid.org/0000-0002-9277-1261)
- William A. Petri (ORCID: https://orcid.org/0000-0002-7268-1218)
- Nicholas Hagspiel
Institutions
- University of Nottingham (GB)
- The Open University (GB)
- University of Virginia Health System (US)
- Nottingham Biomedical Research Centre (GB)
- University of Virginia (US)
- Nottingham Trent University (GB)
Publication Details
- Journal
- Anaerobe
- Published
- 2025-08-09
- DOI
- https://doi.org/10.1016/j.anaerobe.2025.102988
- Primary Topic
- Clostridium difficile and Clostridium perfringens research
- Type
- article
- Field-Weighted Citation Impact
- 0.00