Assessing and avoiding C isotopic contamination artefacts in mesocosm-scale 13CO2/12CO2 labelling systems: from biomass components to purified carbohydrates and dark respiration
The labelling facility enables months-long, quantitative <sup>13</sup>CO<sub>2</sub>/<sup>12</sup>CO<sub>2</sub>-labelling of large numbers of plants with accuracy and precision across contrasts of [CO<sub>2</sub>], empowering eco-physiological study of climate change scenarios. Effective protocols for contamination avoidance are discussed.
Authors
- Regina T. Hirl
- Juan C. Baca Cabrera (ORCID: https://orcid.org/0000-0001-8159-3837)
- Rudi Schäufele (ORCID: https://orcid.org/0000-0001-5288-1397)
- H. Schnyder (ORCID: https://orcid.org/0000-0002-0139-7535)
- Jianjun Zhu (ORCID: https://orcid.org/0009-0006-1394-3667)
Institutions
- Shandong University (CN)
- Forschungszentrum Jülich (DE)
- Sphere Institute (US)
- Technical University of Munich (DE)
Publication Details
- Journal
- Plant Methods
- Published
- 2025-08-11
- DOI
- https://doi.org/10.1186/s13007-025-01431-3
- Primary Topic
- Isotope Analysis in Ecology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Deutsche Forschungsgemeinschaft
- China Scholarship Council
- Technische Universität München