Protocol for recording and analyzing neuronal network activity ex vivo with multi-electrode arrays in mouse brain tissue

Studying neuronal network activity is essential as brain functions arise from dynamic and coordinated interactions between neuronal populations. Here, we present a protocol for recording and analyzing network bursting activity in mouse hippocampal slices. We describe steps for preparing slices and inducing network activity. We then detail the recording procedure with multi-electrode arrays, followed by analysis. This protocol has potential applications for studying different types of network activity in brain tissues from various areas and species, from mice to humans. For complete details on the use and execution of this protocol, please refer to Dossi et al.¹.