Assessment of measurement uncertainty of immunoassays and LC-MS/MS methods for serum 25-hydroxyvitamin D
Measurement uncertainty remains a major challenge for 25-(OH)D assays. The integration of MU-based APS and graphical visualization provides a comprehensive framework for evaluating assay performance. These findings highlight the importance of selecting assays capable of reliably detecting clinically meaningful changes in vitamin D status.
Authors
- Samuel D Vasikaran (ORCID: https://orcid.org/0000-0002-0831-3031)
- Richard Pikner (ORCID: https://orcid.org/0000-0002-0633-8876)
- Pierre Lukas (ORCID: https://orcid.org/0000-0002-1071-9057)
- Callum G. Fraser (ORCID: https://orcid.org/0000-0002-1333-7994)
- Konstantinos Makris (ORCID: https://orcid.org/0000-0002-7896-9028)
- Markus Herrmann (ORCID: https://orcid.org/0000-0002-3559-9899)
- Giovanni Lombardi (ORCID: https://orcid.org/0000-0002-8365-985X)
- Vincent Delatour (ORCID: https://orcid.org/0000-0003-2447-0341)
- Étienne Cavalier (ORCID: https://orcid.org/0000-0003-0947-2226)
- Katleen Van Uytfanghe (ORCID: https://orcid.org/0000-0001-8195-150X)
- Niklas Rye Jørgensen (ORCID: https://orcid.org/0000-0001-9624-5210)
- Harjit Pal Bhattoa (ORCID: https://orcid.org/0000-0002-4909-0065)
- Annemieke C. Heijboer (ORCID: https://orcid.org/0000-0002-6712-9955)
Institutions
- University of Copenhagen (DK)
- University of Debrecen (HU)
- University of Liège (BE)
- University of Dundee (GB)
- National and Kapodistrian University of Athens (GR)
- Medical University of Graz (AT)
- Charles University (CZ)
- Fiona Stanley Hospital (AU)
- Ninewells Hospital (GB)
- Copenhagen University Hospital (DK)
- Rigshospitalet (DK)
- Ghent University (BE)
- Laboratoire National de Métrologie et d'Essais (FR)
- Amsterdam Neuroscience (NL)
- Klatovské Rybářství (Czechia) (CZ)
- IRCCS Ospedale Galeazzi - Sant'Ambrogio (IT)
- Genomics (United Kingdom) (GB)
- Vrije Universiteit Amsterdam (NL)
- University of West Bohemia in Pilsen (CZ)
Publication Details
- Journal
- Clinical Chemistry and Laboratory Medicine (CCLM)
- Published
- 2025-08-11
- DOI
- https://doi.org/10.1515/cclm-2025-0554
- Citations
- 3
- Primary Topic
- Vitamin D Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 4.41