A cell isolation method from Ligusticum chuanxiong Hort. suitable for obtaining high-quality RNA for Smart-seq
This method can yield high-quality trace RNA suitable for Smart-seq analysis. Moreover, the significant differences in the transcriptomes of various small cell clusters types demonstrate the effectiveness and specificity of our manual dissection method.
Authors
- Xu‐Feng Huang (ORCID: https://orcid.org/0000-0002-5895-2253)
- Lan Huang (ORCID: https://orcid.org/0000-0003-1391-3100)
- Xiaofen Liu (ORCID: https://orcid.org/0000-0002-5526-1502)
- Guihua Jiang (ORCID: https://orcid.org/0000-0002-6706-5875)
- Nianyin Zhu
- Mengmeng Wu (ORCID: https://orcid.org/0009-0006-1354-6725)
- S. M. Chen
- Zhiyin Yu (ORCID: https://orcid.org/0000-0001-8048-302X)
- Can Wang (ORCID: https://orcid.org/0000-0002-2157-9889)
- Chi Song (ORCID: https://orcid.org/0000-0003-3904-963X)
- Ruoshi Li (ORCID: https://orcid.org/0000-0003-1943-731X)
- Xianmei Yin (ORCID: https://orcid.org/0000-0003-3891-2067)
Institutions
- Chengdu University of Traditional Chinese Medicine (CN)
Publication Details
- Journal
- Plant Methods
- Published
- 2025-08-10
- DOI
- https://doi.org/10.1186/s13007-025-01425-1
- Citations
- 2
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation
- Natural Science Foundation of Sichuan Province
- Natural Science Foundation for Young Scientists of Shanxi Province
- Young Scientists Fund