Integration of CRISPR/Cas12a and Cas13a in one pot for ratiometric calibration of single-nucleotide variations
Accurate detection of single-nucleotide variations (SNVs) plays a pivotal role in medical diagnosis. Herein, by integrating Cas12a and Cas13a to simultaneously detect wild-type and mutated sites on a single RNA strand, we developed an innovative one-pot SNV analysis method, namely iCasdrop, which is capable of reducing non-specific signals induced by the wild type sequence and achieving ratiometric calibration of SNVs.
Authors
- Yongyuan Zhang (ORCID: https://orcid.org/0000-0001-9337-1553)
- Kaixiang Zhang (ORCID: https://orcid.org/0000-0002-6812-6342)
- Yanan Li (ORCID: https://orcid.org/0000-0002-9528-0273)
- Zhenjie Zheng
- Yige Wu
- Fenglei Quan
- Qingqing Han (ORCID: https://orcid.org/0000-0002-5132-510X)
- Hua Gao (ORCID: https://orcid.org/0000-0002-9698-5830)
- Yuan-Ming Wang (ORCID: https://orcid.org/0000-0002-5612-6003)
Institutions
- Zhengzhou University (CN)
- Beijing Academy of Science and Technology (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc03931b
- Citations
- 1
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Natural Science Foundation of China