Microbiome-mediated colonization resistance to carbapenem-resistant Klebsiella pneumoniae in ICU patients
Carbapenem-resistant Klebsiella pneumoniae (CRKP) causes serious intensive care unit (ICU)-acquired infections, yet the mechanisms of microbiota-mediated colonization resistance remain unclear. We analyzed the gut microbiome and metabolic profiles of healthy individuals and ICU patients, distinguishing those with and without CRKP colonization. ICU patients showed distinct microbial communities compared to healthy controls, and CRKP-positive patients exhibited unique microbial and metabolic signatures. We demonstrated that a healthy gut microbiome is essential for providing resistance against CRKP colonization in antibiotic-perturbed mouse with fecal microbiota transplantation (FMT). Both in vitro and in vivo experiments revealed that Lactiplantibacillus plantarum and Bifidobacterium longum as significant contributors to the decolonization of CRKP. Furthermore, we showed that probiotic supplementation or FMT significantly improved CRKP colonization resistance. The findings highlight that a specific gut microbiome is essential for resisting CRKP colonization, and that targeted microbiome restoration may serve as a viable strategy to prevent CRKP colonization in ICU patients.
Authors
- Yu Feng (ORCID: https://orcid.org/0000-0002-5654-4256)
- Aiping Du
- Yongming Tian (ORCID: https://orcid.org/0000-0001-8440-0324)
- Zhiyong Zong (ORCID: https://orcid.org/0000-0002-7675-260X)
- Xi-Jun Wu (ORCID: https://orcid.org/0000-0001-9576-1328)
- Chenguang Du (ORCID: https://orcid.org/0000-0001-8616-7300)
- Lin Cai (ORCID: https://orcid.org/0009-0008-4491-5380)
- Bairong Shen (ORCID: https://orcid.org/0000-0003-2899-1531)
- Min Jiang (ORCID: https://orcid.org/0000-0002-4646-0067)
- Wei Wu (ORCID: https://orcid.org/0000-0002-0858-8086)
- Huan Liu (ORCID: https://orcid.org/0000-0003-3909-0931)
- Bo Wang (ORCID: https://orcid.org/0000-0002-1048-1034)
- Cong Yan (ORCID: https://orcid.org/0000-0003-4359-9802)
- Chi Zhang (ORCID: https://orcid.org/0009-0000-7838-9114)
- Yumei Li
- Ke Shen (ORCID: https://orcid.org/0000-0003-2818-8124)
- Jing Yang (ORCID: https://orcid.org/0000-0002-0971-4566)
- Yan Kang
- Yi Zhou (ORCID: https://orcid.org/0000-0002-8591-9707)
- Fang Pang
- Zhongwei Zhang (ORCID: https://orcid.org/0009-0004-3909-761X)
Institutions
- Sichuan University (CN)
- West China Hospital of Sichuan University (CN)
Publication Details
- Journal
- npj Biofilms and Microbiomes
- Published
- 2025-08-09
- DOI
- https://doi.org/10.1038/s41522-025-00791-x
- Citations
- 5
- Primary Topic
- Clostridium difficile and Clostridium perfringens research
- Type
- article
- Field-Weighted Citation Impact
- 6.98
Funders
- Sichuan University
- National Key Research and Development Program of China
- West China Hospital, Sichuan University