Induction of autotetraploid and morphological, anatomical, and cytological studies of Trichosanthes cucumerina Linn.

This study successfully induced autotetraploidy in T. cucumerina and established an efficient artificial regeneration system. Compared to diploid plants, autotetraploid plants demonstrated significant improvements in morphological characteristics, anatomical traits, and growth parameters. These findings provide valuable resources for breeding new varieties and advancing genetic research on T. cucumerina.