Enhancing the Yield of Pleurotus ostreatus Through the Addition of Nucleotides and Nucleosides

<i>Pleurotus ostreatus</i> is a mushroom species renowned for its abundant nutritional and medicinal properties. Nevertheless, the yield of its fruiting bodies has long remained at a standstill, making it arduous to achieve substantial improvements. Because the traditional composting approach for enhancing the yield of Pleurotus ostreatus has drawbacks such as a long duration and a high susceptibility to mold contamination, incorporating nutritional supplements into the culture medium of <i>P. ostreatus</i> has emerged as a relatively straightforward yet effective approach to enhancing its yield. This study was predicated on the roles of nucleotides and nucleosides in cellular metabolism and signal transduction. These substances were applied during the cultivation process of <i>P. ostreatus</i> to investigate their impact on the growth and nutritional composition of this mushroom. The findings of this study demonstrate that the supplementation of nucleotides and nucleosides not only improved the yield and biological efficiency of <i>P. ostreatus</i> but also increased its dietary fiber content and amino acids. Furthermore, this research has disclosed that nucleotides and nucleosides exert a notable influence on the lignocellulolytic enzyme system. This investigation provides a scientific foundation for the development of novel yields-enhancing agents for <i>P. ostreatus</i> and offering new insights into cultivation techniques for the progress of <i>P. ostreatus</i> cultivation techniques in both academic and practical arenas.