Determination of Isomatrine in Traditional Mongolian Medicine Agaru-15 Powder by HPLC

A qualitative and quantitative method for the determination of flavonoids in traditional Mongolian medicine agaru-15 powder was established. C18.6 mm chromatographic column is selected × 250 mm, particle size 5 μm. The mobile phase system is acetonitrile water, gradient elution. The detection wavelength was 295 nm. The range of isomatrine was 0.284–2.84 μg. The regression equation is y = 25.811x - 0.1969, r = 0.99990. The recovery was 91.39% and RSD was 1.42%. All the methods mentioned in this paper have the characteristics of simplicity, accuracy, high sensitivity, good reproducibility and strong durability, which provide a reference for the quality control of agaru-15 powder.