“Phytochemical Estimation, Antioxidant and Wound Healing Activity of Polyherbal Gelof Asystasia Gangetica and Euphorbia Cyathophoraextract on Wistar Rats”

This study focuses on the pharmacological assessment of methanolic bark extracts of Asystasiagangetica and Euphorbia cyathophorafor their wound healing potential in Wistar albino rats. The extracts demonstrated considerable yields of 4.90% and 4.25% respectively. Phytochemical screening revealed a rich presence of bioactive compounds such as flavonoids, tannins, triterpenoids, and carbohydrates, which are known contributors to tissue regeneration. Quantitative analysis showed that AsystasiaGangeticacontained higher total phenolic content (92 mg/gm gallic acid equivalent) compared to Euphorbia cyathophora(60 mg/gm), with both extracts exhibiting comparable flavonoid levels (~29 mg/gmrutin equivalent). Herbal gel formulations were developed and evaluated for organoleptic properties, pH, viscosity, and spreadability, all of which fell within acceptable dermal application parameters. The polyherbal gel formulation exhibited superior antimicrobial activity (zone of inhibition: 3 mm) and significantly enhanced wound contraction (80.99 % by day 21), approaching the efficacy of the standard Gentamicin gel (94.74%). These findings suggest a synergistic effect between the two plant extracts and support their traditional use as effective wound healing agents.