Features of microclonal propagation of plants of genus Cercis L.

Due to the complexity of traditional vegetative propagation of Cercis L., it is necessary to use in vitro biotechnology to obtain a regenerative aseptic culture with preservation of decorative features. The study aimed to develop methods of microclonal propagation of Cercis siliquastrum “Alba” and Cercis canadensis L. For this purpose, plant material was collected at different periods of the growing season from plants growing in Kyiv. Statistical and biotechnological methods were used in the study. As a result of the study, two sterilisation modes were tested. The study determined that the efficiency of explant sterilisation and regeneration is influenced by the season of isolation. Using 70% ethyl alcohol and 1% silver nitrate, the sterilisation efficiency of the explants inserted in May was as follows: 20.0±1.8% for Cercis siliquastrum “Alba” and 31.3±3.2% for Cercis canadensis L. The explants were introduced into in vitro culture on hormone-free nutrient medium according to the WPM (Wood Plant Medium) prescription. Aseptic explants were further subcultivation on WPM with 0.4 mg/l BA (N6-Benzyladenine) and 0.3 mg/l 2iP (6-(γ,γ-Dimethylallylamino)purine) and 0.25 mg/l NAA (1-Naphthylacetic acid). The multiplication factor for in vitro shoots on WPM with 0.4 mg/l BA was: 9.4±3.5 for C. canadensis and 9.7±2.9 for C. siliquastrum “Alba”. The use of WPM with 0.3 mg/l 2iP and 0.25 mg/l NAA stimulated active regeneration of in vitro shoots with this multiplication factor: for C. canadensis 5.0±1.5 and C. siliquastrum “Alba” 6.5±1.5. The research obtained shoots and in vitro plants of Cercis L. for further use in the landscaping of settlements