Evaluation of Residues of Benfuracarb and Carbosulfan in Spergularia marina

The residues of benfuracarb and carbosulfan required for insect control in Spergularia marina were evaluated to investigate basic data for their registration with safe use standards.S. marina grown in soil treated with benfuracarb or carbosulfan was harvested and subjected to LC/MS/MS analysis equipped with a modified QuEChERS method for quantitative and qualitative analyses of benfuracarb, carbosulfan and their metabolites carbofuran and 3-hydroxycarbofuran.The methods for sample preparation and instrumental analysis were established to meet the CODEX guidelines.Total residues of benfuracarb were found in the range of 0.01~0.02mg/kg in samples of S. marina grown in soil treated with benfuracarb.The low total levels of benfuracarb were suggested by the rapid degradation of benfuracarb in soil and plant.Total residues of carbosulfan were in the range of 0.02~0.39mg/kg in samples of S. marina grown in soil treated with carbosulfan.Carbosulfan was degraded to carbofuran and 3-hydroxycarbofuran at higher levels than those in benfuracarb-treated samples.The higher total levels of carbosulfan than those of benfuracarb were suggested by an increase in the total amount translocated to plant.The residues of carbosulfan and its metabolites increasing with harvest time at twice the recommended dose suggested continuous uptake and translocation occurred during plant growth.Considering the total residues of benfuracarb and carbosulfan higher than 0.01 mg/kg, the Positive List System level, our study suggests that the establishment of safe use standards for benfuracarb and carbosulfan in S. marina is necessary.