Polymorphism in the BMPR-1B gene and its relationship with lipid metabolism in sheep

The study is devoted to the investigation of the relationship between polymorphism in the bone morphogenetic protein receptor 1B (BMPR-IB) gene and lipid metabolism in Romanov sheep bred in Western Siberia. The study of polymorphisms, including single nucleotide substitutions, is an important aspect in the selection of agricultural animals. They can affect various indicators of animal productivity, disease resistance and adaptation to the environment. This study was conducted on 65 Romanov sheep. DNA analysis was performed by the PCR-RFLP method to identify genotypes for the BMPR-IB gene. For electrophoretic fractionation of DNA samples, 3% gel with the addition of ethidium bromide was used. In addition, the content of heavy metals in the sheep’s habitat was estimated, which did not exceed the MAC. Statistical data processing was performed using Microsoft Office Excel 2007 and RStudio version 1.2.5033. Lipid metabolism was analyzed based on cholesterol, triglycerides, and high-density lipoprotein (HDL) levels. A significant relationship was found between the BMPR1B genotypes and HDL levels. It was found that WW homozygotes had higher HDL levels than WM heterozygotes (p < 0.05). The polymorphism did not affect the blood triglyceride and cholesterol levels in sheep. There are no data in the literature on a direct relationship between BMPR-1B and lipid metabolism. An indirect effect of BMPR-1B is possible through signaling pathways and pleiotropic effects of the gene. Thus, further study of the polymorphism is necessary to clarify the mechanisms of BMPR-1B influence on lipid metabolism.