Influence of succarose concentration on the growth and development of strawberry regenerants

The aim of the study was to optimize the stages of garden strawberry micropropagation. To introduce the Elizaveta 2 variety and the K 10 selection line into in vitro culture, the growth points of the firstorder runners were used as explants using the following sterilization scheme: soap solution; running water; 70% ethanol; 12 % H 2 O 2 . At the stage of micropropagation itself, Murashige-Skoog medium was used with a sucrose concentration of 0, 10, 20, 30 g/l, supplemented with 0.5 mg/l 6-benzylaminopurine (BAP). The final stage of the work included determining the ability of the regenerates to adapt to ex vitro conditions. Vermiculite was used as a substrate. The experiment was repeated three times. The number of roots and microplants from the explant, as well as their viability at the adaptation stage, were recorded. The Elizaveta 2 variety and the K 10 line did not have statistically significant differences in the increase in the number of microshoots. The Elizaveta 2 variety showed a tendency to decrease by 2.03 pcs. (LSD05 = 4.09) in the variant with the addition of 30 g/l sucrose, and the number of roots statistically significantly exceeded the value of the indicator on the control medium (20 g/l sucrose; LSD 05 = 2.64) by 3.23 pcs. On the nutrient medium with the addition of 10 g/l sucrose, the number of roots decreased by 3.77 pcs., and on the medium without sucrose – by 8.38 pcs. The K10 selection line did not form roots in the variant without the addition of sucrose, and no statistically significant deviations were observed in the other variants. Under ex vitro conditions, the micro plants were characterized by 88.8–100 % viability after in vitro cultivation on variants with 20–30 g/l sucrose. Adapted micro plants, cultivated in vitro on a diet containing 30 g/l of sucrose, have a powerful root system (Elizaveta 2 by 6.42 pcs. more, line K 10 by 4.23 pcs., LSD 05 = 4.86), compared to the indicators in the control variant.