Evaluation of the action of myrtenol isolated and in combination with voriconazole and amphotericin B against Aspergillus Niger

This research aimed to evaluate the antifungal potential of the monoterpene myrtenol, both isolated and in combination with the standard antifungals amphotericin B and voriconazole, against strains of <i>Aspergillus niger</i>. The assays for Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), and association using the Checkerboard method were performed. Myrtenol demonstrated strong antifungal activity against all tested strains of <i>A. niger</i>, with a MIC value of 64 µg/mL and an MFC of 256 µg/mL, exhibiting fungicidal action. The combination of myrtenol with amphotericin B resulted in synergy and additivity, while synergy was observed when myrtenol was combined with voriconazole for the tested strain. The obtained results demonstrate the efficacy of myrtenol against <i>Aspergillus</i> in various tests, indicating it as an interesting alternative for the development of a drug with anti-<i>Aspergillus</i> potential.