The <scp>ABAP1 interacting protein</scp> 10 (AIP10) exerts a dual role in the cell cycle and primary metabolism pathways in <i>Arabidopsis thaliana</i>

Plants have developed a sophisticated regulatory network that coordinates gene expression in meristematic zones in response to environmental conditions. Here, we identified a protein in Arabidopsis (Arabidopsis thaliana) that interacts with Armadillo BTB Arabidopsis protein 1 (ABAP1), a negative regulator of the cell cycle in plants. We characterized the ABAP1 interacting protein (named AIP10) investigating its role in modulating plant development. T-DNA insertion lines with silenced expression of AIP10 were evaluated phenotypically (morphology, fresh and dry weight), via transcriptomics analyses (RNA-Seq and RT-qPCR), physiologically (biochemically, Fluorcam and Li-COR) and metabolically (ATR-FTIR). We showed that AIP10 integrates cell division rates with transcriptional and primary metabolism reprogramming through its protein interactions with ABAP1 and KIN10, a subunit of SnRK1 (sucrose non-fermenting-1-related protein kinase 1). ABAP1 levels and activity were reduced in the absence of AIP10, licensing cell cycle progression for longer periods, which culminated in increased rates of cell division that boosted vegetative and reproductive growth. AIP10 knockout triggered a major transcriptional reprogramming of plant primary metabolism, possibly through SnRK1 modulation. aip10 mutants showed increased photosynthetic efficiency, as well as boosted carbon fixation, leading to increased biomass, seed productivity, and higher contents of proteins, lipids (triglycerides), and carbohydrates. Finally, we propose that the modulation of AIP10 expression is part of a mechanism that coordinates higher rates of cell division with better photosynthetic performance and carbon fixation to metabolically meet the plant energy demand, allowing the generation of plants with increased biomass and productivity.

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Publication Details

Journal
The Plant Journal
Published
2025-08-01
DOI
https://doi.org/10.1111/tpj.70399
Primary Topic
Plant nutrient uptake and metabolism
Type
article
Field-Weighted Citation Impact
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article

The ABAP1 interacting protein 10 (AIP10) exerts a dual role in the cell cycle and primary metabolism pathways in Arabidopsis thaliana

Bruna Gino de Araújo‐Lopes, Luíz Mors Cabral, Jelmir Craveiro de Andrade, Joaquin Felipe Roca Paixão et al.
The Plant Journal
Plant nutrient uptake and metabolism
article

The ABAP1 interacting protein 10 (AIP10) exerts a dual role in the cell cycle and primary metabolism pathways in Arabidopsis thaliana

Bruna Gino de Araújo‐Lopes, Luíz Mors Cabral, Jelmir Craveiro de Andrade, Joaquin Felipe Roca Paixão, Helkin Forero Ballesteros, Jérémie Bazin, Carlos Adam Conte‐Júnior, Adriana F. Fusaro, Carinne Moreira de Souza Costa, Eliemar Campostrini, Patrícia Montessoro, Letícia Tessaro, Adriana Silva Hemerly, Letícia Paula Perdigão Grangeiro, Janice de Almeida Engler, Fernanda Silva Coelho, Wallace de Paula Bernardo, Laura Ducatti, Vanessa Iurif, Vivian Ruivo
article en

Abstract

Plants have developed a sophisticated regulatory network that coordinates gene expression in meristematic zones in response to environmental conditions. Here, we identified a protein in Arabidopsis (Arabidopsis thaliana) that interacts with Armadillo BTB Arabidopsis protein 1 (ABAP1), a negative regulator of the cell cycle in plants. We characterized the ABAP1 interacting protein (named AIP10) investigating its role in modulating plant development. T-DNA insertion lines with silenced expression of AIP10 were evaluated phenotypically (morphology, fresh and dry weight), via transcriptomics analyses (RNA-Seq and RT-qPCR), physiologically (biochemically, Fluorcam and Li-COR) and metabolically (ATR-FTIR). We showed that AIP10 integrates cell division rates with transcriptional and primary metabolism reprogramming through its protein interactions with ABAP1 and KIN10, a subunit of SnRK1 (sucrose non-fermenting-1-related protein kinase 1). ABAP1 levels and activity were reduced in the absence of AIP10, licensing cell cycle progression for longer periods, which culminated in increased rates of cell division that boosted vegetative and reproductive growth. AIP10 knockout triggered a major transcriptional reprogramming of plant primary metabolism, possibly through SnRK1 modulation. aip10 mutants showed increased photosynthetic efficiency, as well as boosted carbon fixation, leading to increased biomass, seed productivity, and higher contents of proteins, lipids (triglycerides), and carbohydrates. Finally, we propose that the modulation of AIP10 expression is part of a mechanism that coordinates higher rates of cell division with better photosynthetic performance and carbon fixation to metabolically meet the plant energy demand, allowing the generation of plants with increased biomass and productivity.

The Plant JournalVol. 123(3)
Universidade Federal do Rio de Janeiro (BR), Centre National de la Recherche Scientifique (FR), Universidade Federal Fluminense (BR), Université Côte d'Azur (FR), Université Paris-Saclay (FR), State University of Norte Fluminense (BR), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR), Institut des Sciences des Plantes de Paris Saclay (FR), Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro (BR), Institut Sophia Agrobiotech (FR), Universidade Federal do Estado do Rio de Janeiro (BR)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
Responsible consumption and production
Openalex Percentile: Top 85%
Plant nutrient uptake and metabolism
DOIOpen Access PDFOpenAlex
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