Dehydroxylation/hydroxylation: a novel mechanism for <scp>d</scp>-allulose 3-epimerase-catalyzed ketohexose epimerization was discovered and validated
In response to the disputed dehydrogenation/hydrogenation mechanism of D-allulose 3-epimerase (DAE), this study reveals a dehydroxylation/hydroxylation mechanism <i>via</i><sup>18</sup>O/<sup>2</sup>H isotopic labeling in mass spectrometry and DFT calculations, demonstrating metal-coordinated C3-O3 bond cleavage and providing a basis for the rational modification of DAE in rare sugar biosynthesis.
Authors
- Jidong Shen (ORCID: https://orcid.org/0000-0002-3139-8002)
- Zhi‐Qiang Liu (ORCID: https://orcid.org/0000-0003-3259-6796)
- Shenmin Zhu (ORCID: https://orcid.org/0000-0003-3145-4446)
- Yu‐Guo Zheng (ORCID: https://orcid.org/0000-0002-6358-6243)
- Meng Zhang (ORCID: https://orcid.org/0000-0003-4497-3253)
- Xiaojian Zhang (ORCID: https://orcid.org/0000-0001-6602-3449)
- Baoping Xu (ORCID: https://orcid.org/0000-0001-8938-7691)
- Lianggang Huang
Institutions
- Zhejiang University of Technology (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc02777b
- Citations
- 1
- Primary Topic
- Diet, Metabolism, and Disease
- Type
- article
- Field-Weighted Citation Impact
- 1.29
Funders
- National Key Research and Development Program of China