Serological differentiation between naturally acquired mpox and MVA-BN-vaccine induced antibody responses using ratios of MPXV and VACV antigen pairs in the MSD immunoassay
Our study provides a uniquely broad perspective on orthopox serology by contrasting the gold standard NT and IF methods with the novel MSD multiplex assay and by providing an analysis model of multiplex data to separate previously mpox-infected, MVA-BN-vaccinated, and unexposed individuals.
Authors
- Finn Filén
- Johan Aarum (ORCID: https://orcid.org/0000-0001-9783-0964)
- Victor Yman (ORCID: https://orcid.org/0000-0001-7267-0749)
- Victor Westergren
- Márton Keszei (ORCID: https://orcid.org/0000-0002-1158-2179)
- Wanda Christ (ORCID: https://orcid.org/0000-0003-3886-5248)
- Aleksandra Pettke (ORCID: https://orcid.org/0000-0001-9375-7755)
- Jonas Klingström (ORCID: https://orcid.org/0000-0001-9076-1441)
- Klara Sondén (ORCID: https://orcid.org/0000-0003-1585-8475)
- Sara Gredmark‐Russ (ORCID: https://orcid.org/0000-0002-2446-4323)
- Anna Mia Ekström (ORCID: https://orcid.org/0000-0002-3912-1171)
- Elisabet Storgärd
- Kari Johansen
- Sandra Söderholm
- Núria Mayola Danés
Institutions
- Linköping University (SE)
- Public Health Agency of Sweden (SE)
- Karolinska University Hospital (SE)
- Karolinska Institutet (SE)
- Stockholm South General Hospital (SE)
- Integrated Cardio Metabolic Centre (SE)
- Umeå University (SE)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-08-08
- DOI
- https://doi.org/10.1128/spectrum.00182-25
- Primary Topic
- Poxvirus research and outbreaks
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Svenska Sällskapet för Medicinsk Forskning
- Svenska Läkaresällskapet