Diversity of bradyrhizobial T3SS systems and their roles in symbiosis with peanut ( <i>Arachis hypogaea</i> ) and <i>Vigna</i> species ( <i>V. radiata</i> and <i>V. mungo</i> )

Symbiosis between <i>Bradyrhizobium</i> strains isolated from Lao People's Democratic Republic (Lao PDR) and intercropped legumes (<i>Arachis hypogaea</i>, <i>Vigna radiata</i>, and <i>V. mungo</i>) was regulated by the type III secretion system (T3SS), which delivers effector proteins (T3Es) into host plant cells to modulate nodulation. To explore this mechanism, we sequenced and analyzed seven <i>Bradyrhizobium</i> genomes, identifying putative T3Es across five T3SS groups (G.1-G.5), which were classified based on the sequence of <i>rhcN</i>, a conserved ATPase gene essential for T3SS function. Phylogenetic analysis of <i>rhcN</i> more closely reflected the evolutionary relationships of nodulation genes than those based on 16S rRNA or whole-genome comparisons, underscoring its symbiotic relevance. Functional assays using <i>rhcN</i> mutants revealed group-specific effects on nodulation; G.1 strains showed neutral effects on <i>A. hypogaea</i>, negative effects on <i>V. radiata</i>, and positive effects on <i>V. mungo</i>. G.2 strains consistently promoted nodulation across all hosts and lacked effectors related to SUMO (small ubiquitin-like modifier) pathways, which have been implicated in host defense regulation. G.3 strains reduced nodulation in <i>A. hypogaea</i> but enhanced it in <i>Vigna</i> species. G.4 strains suppressed nodulation in <i>A. hypogaea</i>, and G.5 strains inhibited nodulation across all tested legumes. These findings highlight the diversity in T3SS organization, effector composition, and symbiotic responses among native <i>Bradyrhizobium</i> strains. The identification of known and uncharacterized effectors suggests roles in host compatibility and specificity. These strains, along with their effector profiles, provide a foundation for future functional studies to better understand T3SS-mediated interactions and support the development of targeted inoculants for legume hosts.IMPORTANCEThis study advances our understanding of legume-<i>Bradyrhizobium</i> symbiosis by examining the genetic organization and evolutionary patterns of T3SS genes. Our findings revealed that T3SS gene evolution does not always align with phylogenies based on 16S rRNA or whole-genome sequences, suggesting that horizontal gene transfer and functional adaptation may shape diversification. The observed variation in T3SS architecture and effector profiles among the five distinct <i>Bradyrhizobium</i> groups was correlated with host-specific nodulation outcomes in <i>A. hypogaea</i>, <i>V. radiata</i>, and <i>V. mungo</i>. We also identified novel candidate genes influencing symbiotic signaling and compatibility. These insights into the diversity and function of T3SS components contribute to a broader understanding of host-microbe communication and may support the development of more targeted and efficient rhizobial inoculants for sustainable legume cultivation and improved biological nitrogen fixation.