Production of Recombinant Phage Antibodies Specific to Gentamicin and Their Use in Dot Immunoassay

The potential of using phage display technology to obtain antigentamicin antibodies has been demonstrated. For the first time antigentamicin recombinant antibodies were obtained using a sheep phage library (Griffin.1, United Kingdom). The interaction between the obtained phage antibodies with gentamicin was monitored using a circular dichroism spectrometer. It was shown that the interaction of antigentamicin phage antibodies with the corresponding antibiotic is characterized by the presence of a characteristic exciton doublet: a positive peak at ~233 nm and a more intense negative peak with a maximum at ~240 nm. The possibility of gentamicin indication using a test system based on the dot immunoassay method and antigentamicin recombinant antibodies in aqueous solutions has been demonstrated for the first time; the lower limit of antibiotic detection is 0.5 μg/mL. Using the dot immunoassay method, it was found that antigentamicin phage antibodies did not exhibit activity towards ampicillin and tetracycline but showed activity towards kanamycin (lower limit of detection, 1 μg/mL). The results are promising for further development of methods for gentamicin detection using recombinant phage antibodies.