Registration of three upland cotton germplasm lines resistant to root‐knot and reniform nematodes

Abstract Cotton ( Gossypium hirsutum L.) germplasm lines BARBREN 713‐32‐28 (Reg. no. GP‐1160, PI 706595), BARBREN 713‐32‐30 (Reg. no. GP‐1161, PI 706596), and BARBREN 713‐32‐38 (Reg. no. GP‐1162, PI 706597) were developed and released by the USDA‐ARS in 2024. The objective of the releases was to provide breeders with agronomically elite germplasm that is resistant to both reniform nematode ( Rotylenchulus reniformis Linford & Oliveira) and root‐knot nematode [ Meloidogyne incognita (Kolfoid & White) Chitwood]. The releases also reduce the severity of Fusarium wilt caused by several genotypes of Fusarium oxysporum f. sp. vasinfectum (Fov). The lines have simple sequence repeat (SSR) DNA markers CIR316_202 and BNL3661_185 for the Mi‐1 and Mi‐2 genes for resistance to root‐knot nematode, respectively, and BNL 3279_105 and BNL 569_131 for the Ren 2 GB713 and Ren 3 GB713 genes for resistance to reniform nematode. The single nucleotide polymorphism (SNP) marker GI‐187401 also was associated with the Ren 2 GB713 gene. In controlled environment assays, these lines suppressed numbers of reniform nematode eggs by a mean of 83.6% and root‐knot nematode eggs by a mean of 97.2% compared to control plants. The released lines with both Mi‐1 and Mi‐2 genes had significantly greater shoot weight than other experimental lines with the Mi‐1 gene alone, but not the Mi‐2 gene in soils infested with both root‐knot nematode and several genotypes of Fov race 1. The lines outyielded Fibermax ‘FM 966’ by 12% to 20% and had fiber quality equal to or greater than Phytogen ‘PSC 355’.