Tiled PCR Amplification for Poliovirus Whole-Genome Sequencing v1

This protocol describes the amplification and whole-genome sequencing of poliovirus from clinical or environmental RNA extracts using an overlapping tiled amplicon strategy. Four overlapping PCR products are generated from viral RNA via RT-PCR. Amplicons are pooled and purified before library preparation for Oxford Nanopore or Illumina sequencing. This protocol enables robust recovery of full poliovirus genomes for surveillance and research purposes.