Whole-Genome Sequencing and Comparative Genomic Analysis of a Selenium-Enriched Saccharomyces cerevisiae Strain

Selenium enrichment usually refers to the ability of organisms to absorb and accumulate selenium, which may be used in their own metabolic processes or as a storage. Saccharomyces cerevisiae has a rich biological enzyme conversion system, which can convert inorganic selenium into highly active organic selenium and synthesize a variety of probiotic nutrients. In this study, a strain preserved in the laboratory, named W303, was used for whole-genome sequencing to preliminarily explore the mechanism of selenium enrichment performance of this strain, aiming to provide a feasible reference for subsequent studies on the specific enhancement and application of selenium-enriched yeast. High-quality sequencing data were obtained through Illumina sequencing technology, and a strict quality control process was carried out to ensure the accuracy and reliability of the data. The results of genome assembly showed that the genome size of W303was approximately 11.71 Mb, the N50 was 41.72 kb, and the GC content was 38.66%. A total of 6337 coding genes, 213 tRNAs, and 3 rRNAs were obtained through gene element prediction. Gene function annotation covered multiple databases such as NR, GO, and KEGG, with an annotation success rate of 92.3%. A phylogenetic tree based on ITS was constructed. Pan-genome analysis revealed the distribution of core genes, accessory genes, and unique genes. This study provides comprehensive data support for a deep understanding of the genomic characteristics, functional genes, and evolution of this fungus.