Distinct microRNA signatures define sporadic PSP-RS and PD in patient-derived midbrain organoids
Progressive supranuclear Palsy-Richardson syndrome (PSP-RS) is a rare, rapidly progressive tauopathy often misdiagnosed as Parkinson's disease (PD) due to overlapping clinical features and the lack of reliable molecular biomarkers. To address this need, we generated human midbrain organoids from induced pluripotent stem cells (iPSCs) derived from individuals with sporadic PSP-RS, PD, and healthy controls (HCs), and performed longitudinal small RNA sequencing to profile microRNA (miRNA) signatures. These 3D organoids recapitulated disease-relevant pathologies, including tau hyperphosphorylation in PSP-RS and α-synuclein aggregation in PD. Transcriptomic analysis revealed dynamic, disease-specific miRNA signatures. Notably, miR-5683, miR-873-5p, miR-219b-5p, and miR-219a-2-3p were enriched in PSP-RS, while PD organoids showed increased expression of miR-1-3p and miR-133b. Differential expression analysis identified miR-5683, miR-3085-3p, and miR-124-3p as robust classifiers distinguishing PSP-RS from controls. Our findings support iPSC-derived midbrain organoids as a relevant platform for modeling atypical parkinsonian syndromes and uncovering candidate miRNA biomarkers for early and differential diagnosis.
Authors
- Andrea Quattrone (ORCID: https://orcid.org/0000-0003-2071-2083)
- Graziano Pesole (ORCID: https://orcid.org/0000-0003-3663-0859)
- Giovanni Cuda (ORCID: https://orcid.org/0000-0001-6313-1866)
- Aldo Quattrone (ORCID: https://orcid.org/0000-0003-2001-957X)
- Stefania Scalise (ORCID: https://orcid.org/0000-0001-6979-1486)
- Fulvio Chiacchiera (ORCID: https://orcid.org/0000-0003-3830-2090)
- Elvira Immacolata Parrotta (ORCID: https://orcid.org/0000-0001-8145-3623)
- Clara Zannino (ORCID: https://orcid.org/0000-0003-1919-3212)
- Mariagrazia Talarico (ORCID: https://orcid.org/0000-0002-7439-3973)
- Ermes Filomena
- Giorgia Lucia Benedetto
- Valeria Lucchino (ORCID: https://orcid.org/0000-0002-7350-3835)
- Davide Bressan (ORCID: https://orcid.org/0000-0003-4757-2535)
- Desirèe Valente
- Ida Lazzinnaro
Institutions
- University of Trento (IT)
- Magna Graecia University (IT)
- Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies (IT)
- National Research Council (IT)
- University of Bari Aldo Moro (IT)
Publication Details
- Journal
- iScience
- Published
- 2025-07-18
- DOI
- https://doi.org/10.1016/j.isci.2025.113162
- Citations
- 1
- Primary Topic
- Cancer-related molecular mechanisms research
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- Alliance for Cancer Gene Therapy
- British Computer Society
- Centro universitario di ricerca e formazione per lo sviluppo competitivo delle imprese del settore vitivinicolo italiano, Università degli Studi di Firenze