Optimizing chromosome dispersion quality: the key role of cell density.
This study innovatively established a turbidity-based cell density adjustment method, clarifying the impact of cell density on metaphase dispersion through quantitative means and providing standardized technical support for automated detection. This method effectively addresses the inconsistency in metaphase dispersion due to varying cell densities in automated detection, offering a significant basis for homogenizing detection results across laboratories and advancing the standardization and homogenization of chromosomal aberrations analysis techniques.
Authors
- Chao-xian Gao
- Chang-ye Hui (ORCID: https://orcid.org/0000-0002-0958-1986)
- Boxin Li (ORCID: https://orcid.org/0000-0003-0037-2392)
- Yuting Chen (ORCID: https://orcid.org/0000-0003-4579-6498)
- Yuming Guo (ORCID: https://orcid.org/0000-0002-1766-6592)
- Limei Li (ORCID: https://orcid.org/0000-0002-2728-5072)
- Xueqin Yang (ORCID: https://orcid.org/0000-0002-3638-2720)
Institutions
- Shenzhen Occupational Disease Prevention Hospital (CN)
- Shenzhen Center for Disease Control and Prevention (CN)
Publication Details
- Journal
- PubMed
- Published
- 2025-01-01
- DOI
- https://doi.org/10.3389/fcell.2025.1636498
- Primary Topic
- Molecular Biology Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Natural Science Foundation of Shenzhen City