Field Sampling of Zaprionus indianus in the Eastern United States in 2023-2024

We conducted surveys for the invasive drosophilid <i>Zaprionus indianus</i> in the eastern United States in 2023 and 2024. We found no latitudinal trends in <i>Z. indianus</i> abundance, and the northern boundary for <i>Z. indianus</i> was variable between years. <i>Z. indianus</i> was rare in central and northern Florida in the spring, was not present on early season berry crops in Virginia, and had a restricted temperate growing season compared to other drosophilids, providing further support for the species' yearly recolonization of temperate areas. <i>Z. indianus</i> was also observed on native fruits in a natural area in Virginia.