Method of Confirmation and Predictive Metabolomics: A Novel Multidimensional Analytical Framework for Characterizing New Psychoactive Substances

The validated UPLC-QTOF-MS method provides a powerful, reliable tool for the rapid detection and confirmation of emerging NPS in forensic samples. Its high mass accuracy, ability to perform non-targeted analysis, and support for retrospective interrogation make it a superior alternative to traditional mass spectrometric methods. This approach enhances forensic laboratories' ability to keep pace with evolving drug trends and strengthens the integrity of drug-related investigations.